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. 2016 Feb 24;14:57. doi: 10.1186/s12967-016-0815-y

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© Liu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The accumulation of DN T cells in the pancreas-associated LN and the spleen as opposed to the MLN 6 months after combined ATS and DN T cell treatment. The spleen, pancreatic draining LN and mesenteric LN were harvested from different NOD mice and single cell suspensions were prepared. The percentage of CD4⁺, CD8⁺ and DN T cells in CD3+TCR-β⁺ T cells were examined by flow cytometry