Table 1.
Primers for amplifying mouse cytokine cDNA by RT-PCR
| Gene | Forward primer sequence (5′ → 3′) | Reverse primer sequence (5′ → 3′) | Accession No. | Size/bp |
|---|---|---|---|---|
| GAPDH | GAAGGGTGGAGCCAAAAGGGTCAT | CATTGGGGGTAGGAACACGGAAGG | GU214026.1 | 360 |
| IL-1β | TACAGGCTCCGAGATGAACAACAA | TGGGGAACTCTGCAGACTCAAACT | BC011437.1 | 332 |
| TNFα | AGGGGCCACCACGCTCTTCTGT | GCAAATCGGCTGACGGTGTGG | BC117057.1 | 358 |
| IL-6 | AACCACGGCCTTCCCTACTTC | TCTGGCTTTGTCTTTCTTGTTATC | NM_031168.1 | 383 |
| IL-8 | ATGGCTGCTCAAGGCTGGTC | AGGCTTTTCATGCTCAACACTAT | NM_011339.2 | 386 |
| MCP-1 | AGGTCCCTGTCATGCTTCTGG | TTACGGGTCAACTTCACATTCAAA | BC145867.1 | 367 |