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. 2016 Feb 24;16:4. doi: 10.1186/s12861-016-0106-0

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© Gurdziel et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Functional verification of GLI-dependent enhancer activity. Putative regulatory regions were cloned upstream of a minimal promoter co-transfected into C2C12 cells, along with a GLI1 expression vector. Relative activity is plotted (stimulated/basal). The Ptch2 region is used as a positive control. Novel regions annotated to the Boc, Dpp6, Hhip, Hipk2, Ptch1, Scube1, and Tgfbr2 loci exhibit upregulation in response to GLI1 co-transfection (green). Dependence on GLI was assessed by mutating all putative GLI TFBS (GKO) within the sequence and retesting in the assay (gray)