Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 24;35:36. doi: 10.1186/s13046-016-0310-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Dorward et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — AQP1 expression. (a) relative expression of AQP1 (qPCR) in HUVEC, colon cancer cell lines, and human colon tumours (error bars show mean ± SEM; ** p = 0.004 ANOVA); (b) relative expression of AQP1 in CRC patients’ tumours compared to their matched normal mucosa. (c) western blot showing AQP1 monomer: lane 1 HUVEC, lane 2 HCT-116, lane 3 HT29. (d) corresponding quantification of bands using Image Lab™ Software (Bio-Rad); (e) immunofluorescence (IF) of HT29 colon cancer cells stained with anti-AQP1 and goat anti-rabbit secondary–Alexa 468 conjugate (orange) with NucBlue® stained nuclei overlay (63 x objective, scale bar = 10 μm). (f, g, h) immunohistochemistry of colon tumour sections staining for AQP1: F low, G moderate, H high expression. Arrows show examples of strong AQP1 staining of microvessels (20 x objective, scale bar = 0.1 mm)