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. 2016 Feb 24;16:76. doi: 10.1186/s12906-016-1055-7

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© Yusof et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Phase contrast micrographs of corneal keratocytes cultured in four different media. a Basal medium (BM), (b) BM + 0.0015 % GH, (c) Basal medium with serum (BMS) and (d) BMS + 0.0015 % GH. Micrographs were taken at Passage 1 Day 3 of culture. Cell density was the least in BM medium (a) and the highest in BMS + 0.0015 % GH (d). Corneal keratocytes cultured in BM only (a) and with GH (b) exhibited shorter and broader cell morphology (thin arrow) compared to that of elongated and fibroblastic-shaped cells (thick arrow) in BMS medium (c) and with GH (d). Magnification (x50)