Table 2.
Quantitative group comparisons using ANOSIM to analyze small effect sizes (within environments) of simulated human saliva and public restroom floor samples
| Test statistic (R) | p value | |
|---|---|---|
| Restroom samples | ||
| Non-phylogenetic methods | ||
| Jaccard to analyze FTSCs | 0.225 | 0.001 |
| Bray-Curtis to analyze FTSCs | 0.239 | 0.001 |
| Jaccard to analyze GTSCs | 0.053 | 0.001 |
| Bray-Curtis to analyze GTSCs | 0.056 | 0.001 |
| FastTree | ||
| Unweighted UniFrac with FastTree to analyze FTSCs | 0.673 | 0.001 |
| Weighted UniFrac with FastTree to analyze FTSCs | 0.798 | 0.001 |
| Unweighted UniFrac with FastTree to analyze GTSCs | 0.038 | 0.057 |
| Weighted UniFrac with FastTree to analyze GTSCs | -0.001 | 0.518 |
| ghost-tree | ||
| Unweighted UniFrac with ghost-tree to analyze FTSCs | 0.125 | 0.001 |
| Weighted UniFrac with ghost-tree to analyze FTSCs | 0.073 | 0.001 |
| Unweighted UniFrac with ghost-tree to analyze GTSCs | 0.619 | 0.001 |
| Weighted UniFrac with ghost-tree to analyze GTSCs | 0.655 | 0.001 |
| Saliva samples | ||
| Non-phylogenetic methods | ||
| Jaccard to analyze FTSCs | 0.250 | 0.001 |
| Bray-Curtis to analyze FTSCs | 0.253 | 0.001 |
| Jaccard to analyze GTSCs | 0.032 | 0.001 |
| Bray-Curtis to analyze GTSCs | 0.032 | 0.001 |
| FastTree | ||
| Unweighted UniFrac with FastTree to analyze FTSCs | 0.852 | 0.001 |
| Weighted UniFrac with FastTree to analyze FTSCs | 0.756 | 0.001 |
| Unweighted UniFrac with FastTree to analyze GTSCs | 0.031 | 0.001 |
| Weighted UniFrac with FastTree to analyze GTSCs | 0.023 | 0.001 |
| ghost-tree | ||
| Unweighted UniFrac with ghost-tree to analyze FTSCs | 0.125 | 0.001 |
| Weighted UniFrac with ghost-tree to analyze FTSCs | 0.068 | 0.001 |
| Unweighted UniFrac with ghost-tree to analyze GTSCs | 0.524 | 0.001 |
| Weighted UniFrac with ghost-tree to analyze GTSCs | 0.596 | 0.001 |
Note: For restroom sample diversity metrics, sample size is 160, and 16 groups were analyzed using 999 permutations. For saliva sample diversity metrics, sample size is 200, and 20 groups were analyzed using 999 permutations