Fig. 1.

Sorafenib and axitinib can overcome TKI resistance. a Overview of resistance mechanisms in CML. b/c KBM5 and KBM5-T315I cells were exposed to the indicated inhibitors or DMSO for 48 h. Cells were stained with 7-AAD and assessed for viability (b) or metabolic activity (MTT assay) (c). d KBM5 and KBM5-T315I cells were exposed to the indicated inhibitors or DMSO for 4 h. Cells were analyzed by western blotting using the indicated antibodies. e/f K562tet Gab2 cells, exposed to 1 μg/ml doxycycline 48 h prior to the treatment or non-induced cells were treated with the indicated inhibitors or DMSO for 72 h. Cells were stained with PI and assessed for viability (e) or metabolic activity (MTT-assay) (f). g K562tet Vector and Gab2 cells were exposed to 1 μg/ml doxycycline 48 h prior to the treatment with the indicated inhibitors or DMSO for 4 h. Cells were analyzed by western blotting using the indicated antibodies. Relevant statistically significant effects are indicated by asterisks, all statistical data can be found in the supplement (b/c/e/f; Additional file 8 )