Fig. 9.

Inositol phosphate levels in maize RNAi lines. a RT-PCR of Mf3 in maize RNAi lines. E1, E2 and E3, different transgenic events/lines; WT, wild-type, negative lines segregated from transgenic lines; GAPDH, glyceraldehyde 3-phosphate dehydrogenase gene (used as a reference gene). b IP levels in seeds of maize transgenic lines. E1, E2 and E3 represent RNAi transgenic lines. **, significant differences between E1, E2 or E3 and WT (negative transgenic lines) were determined using Student’s t-test (p < 0.01)