Table 1.

qPCR primer design and conditions for tissue distribution analysis.

	Primer Sequence
mABHD1	F: 5’ – GGG TAC AGA ACG AAC CGA AA – 3’ R: 5’ – AGA GAA CAG GGG TGT GGA TG – 3’
mABHD2	F: 5’ – TTG ACA TGG CTT TGT GTG GT – 3’ R: 5’ – CGA CAT GGT GAT GAA CTT GC – 3’
mABHD3	F: 5’ – AGT TTC AGC CGC TTC CTA CA – 3’ R: 5’ – GAG ATC TGT CCT CCG TCT GC – 3’
mABHD4	F: 5’ – GTT CCA ATC CAC TGG CTG TT – 3’ R: 5’ – AGG ACT CCA TCA TGG CTT TG – 3’
mABHD5	F: 5’ – TGA CAG TGA TGC GGA AGA AG – 3’ R: 5’ – AGA TCT GGT CGC TCA GGA AA – 3’
mABHD6	F: 5’ – GAC ACA AGC CAT CCA TCC TT – 3’ R: 5’ – ACT TGC CCC ACT ATG GAC AG – 3’
mABHD7	F: 5’ – TCC AAA GGG AAT ACC CAC AA – 3’ R: 5’ – CAG GTG CAT CAG ACT CTC CA – 3’
mABHD8	F: 5’ – GGC TAC ACA TTC GTG GAG GT – 3’ R: 5’ – GTG GGC CAG GAA ACT ACA GA -3’
mABHD9	F: 5’ – GGC GAG CAT TGC TTT CTA AC – 3’ R: 5’ – TCC ACT TCC TTT GGA GCA TC – 3’
mABHD10	F: 5’ – CCT ACG CTG ACG ACT GAT GA – 3’ R: 5’ – TGA TGC CTT TTG TCT GCA AG – 3’
mABHD11	F: 5’ – GCC CTA GGA AGC ACA CTT TG – 3’ R: 5’ – GTC CTG ACT CAT GGC CTC AT – 3’
mABHD12	F: 5’ – TCA TCC TGA CAG GTG CTG AG – 3’ R: 5’ – ACC AGA TTT GTT GCC ACT CC – 3’
mABHD13	F: 5’ – AGG CCA ATA TGC TGT GGA AG – 3’ R: 5’ – GGC CAG TAG CCA TCT TTC AA – 3’
mABHD14a	F: 5’ – ACC ACC AGC TAC GTG GAT TC – 3’ R: 5’ – ATC ATG CAG CTT CAC CAC AG – 3’
mABHD14b	F: 5’ – AGG GCC AGA ACC TCT TCT TC – 3’ R: 5’ – ATC GCC CAA GAC CAT TAC AG – 3’
mABHD15	F: 5’ – TCT TGC TGC ACC AGA AAA TC – 3’ R: 5’ – CGG TGT CCA CTG TGT CCT C – 3’
mABHD16a	F: 5’ – ACA TTG CTG CTG CTA CTT GC – 3’ R: 5’ – GTA CTG GGG GTT GGT CCA G – 3’
mABHD16b	F: 5’ – AGT GCC TCC TCC AGC AGA T – 3’ R: 5’ – TGA GTG GGC CAG TGC ATA G – 3’

RNA was extracted from ~100-400mg of tissue in Trizol (Invitrogen). 1μg of RNA was reverse transcribed using omniscript RT (Qiagen) to generate cDNA. Relative mRNA was measured by quantitative real-time PCR using the delta-delta-CT method. Liver was set to 1 for all ABHD profiles.