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. Author manuscript; available in PMC: 2016 Mar 15.
Published in final edited form as: Dev Biol. 2015 Jan 13;399(2):237–248. doi: 10.1016/j.ydbio.2014.12.034

Figure 1. cftr is expressed in the pancreatic duct.

Figure 1

(A–D) Wholemount in situ hybridization for cftr. (A) Dorsal and (B) lateral view of cftr expression at 3 dpf. (C) Dorsal and (D) lateral view of cftr expression at 5 dpf. (E,F) Transverse section of TgBAC(cftr-GFP) expressed in the pancreatic duct at (E) 3 dpf in conjunction with ptf1a expression in the acinar cells of the pancreas and (F) at 5 dpf in conjunction with ZN-5, an antibody that marks the pancreas. (G) Live, confocal image of TgBAC(cftr-GFP) expression along the length of the pancreatic duct in conjunction with Tg(ins:dsRed) to mark the β cells of the principal islet at 5 dpf. (H) Live confocal image of ductal TgBAC(cftr-RFP) expression in conjunction with ela:GFP to indicate the acinar tissue. White arrows indicate cftr expression in the pancreatic duct. ib: intestinal bulb, ov: otic vesicle, p: pancreas. (A–D) Scale bars = 100 µm, (E–H) Scale bars = 50 µm.