Figure 6. Neutrophil recruitment during pancreatic destruction.

(A,B) Neutrophils are absent from representative sections of (A) WT and (B) cftr mutant pancreata at 14 dpf. (C,D) Neutrophils are absent from the (C) WT pancreas at 16 dpf, but present in the (D) cftr mutant pancreas. (E,F) Quantification of (E) the ratio of sections with neutrophils present versus absent to the total number of sections and (F) the average number of neutrophils observed in each pancreas indicating a significant difference in pancreatic neutrophils in the 16 dpf cftr mutant pancreas. The median number of neutrophils in each WT pancreas was 0, ranging from 0 to 1 and cftr^pd1049 was 1, ranging from 0 to 5. WT, n=8; cftr^pd1049, n=13; *P<0.01. Error bars represent s.e.m. (G,H) DMSO treated (G) WT and (H) cftr mutant samples at 18 dpf expressing ela:GFP in the acinar cells demonstrate typical pancreatic destruction. (I,J) Representative sections of (I) WT and (J) cftr mutant pancreata at 18 dpf treated with PF1052 showing preservation of acinar tissue. (K) Quantification of area of GFP expression divided by total pancreas area for each treatment group showings significantly more area of acinar tissue marked by ela:GFP in PF1052 treated mutants compared with DMSO treated mutants. WT DMSO, n=8; WT PF1052, n=7; cftr^pd1049 DMSO, n=8; cftr^pd1049, n=12; *P<0.01. Error bars represent s.e.m. Arrows indicate neutrophils. Scale bars = 50 µm.