Fig 3. Complementation of the sensitivity to external oxidants of the grx yeast mutants by the R. irregularis GRX genes.

A. Effect of RiGRX4, RiGRX5 and RiGRX6 expression on the sensitivity of the Δgrx3Δgrx4 strain to 1 mM hydrogen peroxide (H₂O₂). B. Effect of RiGRX4, RiGRX5 and RiGRX6 expression on the sensitivity of the Δgrx5 strain to 0.1 mM menadione (Md). The photographs were taken after 3 days of growth at 30°C. C. Effect of RiGRX6 expression on the sensitivity of Δgrx6Δgrx7 to 500 mM CaCl₂ (40 h). Data are means of three independent experiments +/- standard error and represent the growth yield ratio between treated and untreated cultures and then made relative to this ratio in cells expressing the S. cerevisiae Grx6. Asterisks show statistically significant differences (p<0.05) relative to the strain transformed with the empty vector, according to the Fisher’s LSD test.