Fig 1. Targeted degradation is achievable using N-terminal fusions and elucidated with model-driven analysis.

(A) A cleavable tag is fused N-terminally to the target protein. On cleavage, an N-degron is exposed leading to degradation of the target. (B) Balanced exponential phase growth experiments can be used to estimate parameters for a quantitative model (C) to calculate degradation rates. See S5 Fig for a detailed demonstration of calculations.