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. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746

Fig 3. The Ntag confers ClpP-dependent degradation of RFP and beta-galactosidase.

Fig 3

Measuring the apparent degradation rate (β) for the Ntag-RFP construct was significantly higher in the wildtype versus ΔclpP strain (A). For an untagged RFP, there is no appreciable degradation evident by the lack of apparent degradation rate (B). Fusing Ntag to beta-galactosidase resulted in similar degradation as measured by a significant β value (C). For all calculations of the apparent degradation rate, the respective ΔclpP condition is used to calculate the synthesis rate (α). This methodology is illustrated in S5 Fig. Single * designates p < 0.05 and triple (***) designates p < 0.001. All p values are calculated between ΔclpP and WT strains for given constructs. Values are mean ± s.e.m. (n = 4 to 10).