Fig 5. Ntag degradation is enhanced by ClpP and ClpS overexpression.

Overexpression of a perturbation protein was used to test for degradation effects on Ntag-RFP. Overall degradation is reduced with the target/perturbation plasmid system as indicated by the control conditions (ΔclpP + pTrc versus WT + pTrc). ClpA overexpression did not change degradation detectably. Overexpression of each ClpP and ClpS enhanced the degradation rate. Single * designates p < 0.05 and triple (***) designates p < 0.001. Calculations of p-values are between ΔclpP + pTrc and the given condition. Values are mean ± s.e.m. (n = 4 to 6).