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. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746

Fig 6. Degradation rate is minor for protein knockdown in rapidly growing cells.

Fig 6

The target/perturbation plasmid system converts to an inducible, dynamic knockdown platform by temporally expressing ClpP within a clpP deletion strain (A). In high growth rate, knockdown is predicted to be primarily a result of dilution and degradation only slightly increases knockdown compared to synthesis repression alone (B). Experimental inducibility is tested using the target/perturbation system with Ntag-RFP as the target (C). β, degradation, increases post induction (D). Single * designates p < 0.05. All experimental values are mean ± s.e.m. (n = 6 to 8).