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. 2015 Sep 28;66(22):7377–7389. doi: 10.1093/jxb/erv434

Fig. 3.

Fig. 3.

Relative transcript expression and regulation of StWRKY1 by sHSP17.8. The relative transcript expression for (A) StWRKY1 and (B) sHSP17.8 was confirmed in the resistant genotype relative to the susceptible genotype following P. infestans and mock-solution inoculation at 48 hpi using qRT-PCR in comparison with reference genes for elongation factor-1α and β-tubulin. See main text for further details. Regulation of StWRKY1 by sHSP17.8 was confirmed by LUC transient expression assay: (C) constructs used and (D) relative LUC reporter activity by HSP17.8. Values are averages of three replicates. Significant differences in relative expression levels using Student’s t-test: *P<0.05; **P<0.01.