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. 2016 Feb 24;11(2):e0150040. doi: 10.1371/journal.pone.0150040

Table 1. Behaviours considered by participants as sufficient (reliable) for pain and their presence in high and/or low level pain.

Behaviour sufficient for pain Presence in low level pain Presence in high level pain Participant comments
Lameness Frequent Frequent
Difficulty to jump Frequent Frequent
Abnormal gait Frequent Frequent Can be provoked by other conditions: e.g. cerebellar hypoplasia
Reluctant to move Frequent Frequent
Reaction to palpation Frequent Frequent
Withdraw/hiding Frequent Frequent
Absence of grooming Frequent Frequent
Playing less Frequent Frequent
Appetite decrease Frequent Frequent
Overall activity decrease Frequent Frequent
Less rubbing toward people Frequent Frequent
General mood 1 Frequent Frequent
Temperament 2 Frequent Frequent
Hunched up posture Frequent Frequent
Shifting of weight Frequent Frequent It is relatively subjective
Licking a particular body region Frequent Frequent
Lower head posture Frequent Frequent
Blepharospasm* Frequent Frequent Caused by any chronic eye disease
Change in form of feeding behaviour Rare Frequent Require extensive knowledge of prior feeding behaviour. Not reliable to pain
Avoiding bright areas Rare Frequent Any disease of the eyes can cause it
Growling Rare Frequent More useful if it is a new behaviour, related to mood
Groaning Rare Frequent Not reliable sign of pain
Eyes closed Rare Frequent Other possible causes for it (not specified)

* this behaviour was considered reliable for an acute condition.

1 Mood states: i.e., enduring episodic changes in underlying affective predisposition arising as a result of a series of emotional events of congruent emotional valence, for example a tendency to be irritable from time to time as a result of pain25

2 Temperament, i.e., a general disposition or trait that is consistent across time and contexts. This indicates that the pain is persistent, or relief is only temporary, and that a state of pain has become an integral part of the animal’s constitution and its behavioural predispositions shifted accordingly to adapt to the impact of this. For example, a cat in chronic pain might be described as jumpy or irritable the whole time25.