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. Author manuscript; available in PMC: 2016 Feb 24.
Published in final edited form as: Plant J. 2011 Jun 21;67(5):763–773. doi: 10.1111/j.1365-313X.2011.04630.x

Figure 2. Effect of mutations in the CCT domain on the strength of protein interactions between ZCCT and NF-Y proteins.

Figure 2

The mutations include A) ZCCT1-R35W and B) ZCCT1-R39C, C) ZCCT2-R16C, and D) ZCCT2-R16C+ R39C. The ZCCT wild type (grey bars) and mutant (black bars) proteins were expressed as DNA-binding domain fusions and the NF-Y proteins as activation domain fusions. The α-gal assays were used to quantify the strength of the interactions. Relative α-gal activity values for each interaction are the average of 10-16 replicates (error bars = s.e.m. and * = P< 0.01). The insets show the α-gal activity level for ZCCT2-NF-YB3 interaction using a different scale.