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. Author manuscript; available in PMC: 2016 Feb 24.
Published in final edited form as: Plant J. 2011 Jun 21;67(5):763–773. doi: 10.1111/j.1365-313X.2011.04630.x

Figure 3. Competition of ZCCT1 and CO2 proteins for interactions with NF-Y proteins.

Figure 3

Yeast three-hybrid assays were used to test A) the effect of ZCCT1 as competitor when CO2 was expressed as the DNA-binding domain fusion, and B) the effect of CO2 as competitor when ZCCT1 was expressed as the DNA-binding domain fusion (NF-Y proteins were expressed as activation domain fusions). The α-galactosidase activity of the protein interactions in the absence of the competitor is shown in grey bars, and in the presence of the competitor in black bars. Relative α-gal activity values for each interaction are the average of 10-12 replicates (error bars= s.e.m. and * = P< 0.01). The insets show the α-gal activity for ZCCT1-NF-YB3 and ZCCT1-NF-YC10 interactions using a different scale.