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. Author manuscript; available in PMC: 2016 Feb 24.
Published in final edited form as: Plant J. 2011 Jun 21;67(5):763–773. doi: 10.1111/j.1365-313X.2011.04630.x

Figure 4. Effect of the CCT domain mutation R35W on ZCCT1-NF-Y interactions and on competition between ZCCT1 with CO2.

Figure 4

The α-gal assays were used to quantify the relative strength of the protein interactions. Relative α-gal activity values for each interaction are the average of 10-16 replicates. Percentages indicate the percent reduction in α-gal activity. A) Effect of the ZCCT1-wild type protein expressed as a competitor in Y3H assays on the strength of the interactions between CO2 (DNA-binding domain fusion) and NF-YB1, NF-YB3 and NF-YC11 (activation domain fusions ), B) Effect of ZCCT1-R35W mutant protein expressed as a competitor in Y3H assays on the strength of the interaction between CO2 (DNA-binding domain fusion ) and NF-YB1, NF-YB3 and NF-YC11 (activation domain fusions).