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. Author manuscript; available in PMC: 2016 Aug 25.
Published in final edited form as: Connect Tissue Res. 2015 Aug 25;56(5):392–402. doi: 10.3109/03008207.2015.1066781

Figure 5. Pericyte motility is observed along EC tubes within vascular guidance tunnels in 3D matrices.

Figure 5

(A) mRFP-labeled ECs and GFP-pericytes were co-cultured and videos were made of EC tubulogenesis, pericyte recruitment and pericyte motility along EC tubes over time. Still images from these videos are shown at the indicated times of culture and reveal marked pericyte motility along the abluminal EC tube surface. Note that EC tube remodeling is also occurring while pericytes are moving along the tubes. Bar equals 50 μm. (B) EC and GFP-pericytes were co-cultured and after 120 hr, cultures were fixed and stained with anti-CD31 and anti-collagen type I (Col I) antibodies. Note that pericytes are observed on the EC tube abluminal surface and this recruitment is observed within vascular guidance tunnels (arrowheads indicate the borders of these tunnels), which are generated as a result of the EC tubulogenic process. Bar equals 50 μm.