FIGURE 3.

Effect of iron on the intracellular calcium of K562 cells. Iron uptake regulates intracellular Ca²⁺ levels, K562 cells without desferrioxamine (DFO) (A) and with DFO (100 µM) (B) were loaded with Furo-2, (C) shows [Ca²⁺]i of Furo-2 loaded K562 cells in presence of thapsigargin (1 µΜ) at various concentrations of iron in K562 cells without DFO and with DFO (D), (E) The effect of ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA). After treatment with EGTA to Furo-2-loaded K562 cells, [Ca²⁺]i dropped with the addition of iron, (F) [Ca²⁺]i increased in K562 cells without DFO after addition of iron in presence of 2-aminoethoxydiphenyl borate (2-APB), ryanodine and thapsigargin, (G) [Ca²⁺]i did not increase in K562 cells treated with DFO after addition of iron in presence of 2-APB, ryanodine and thapsigargin.