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. Author manuscript; available in PMC: 2016 Feb 24.
Published in final edited form as: Methods Mol Biol. 2015;1250:363–376. doi: 10.1007/978-1-4939-2074-7_28

Fig. 1.

Fig. 1

Bile acid-induced necrotic cell death in murine hepatocytes. Hepatocytes were isolated from livers of C57Bl/6 mice and exposed to various concentrations of glycocholic acid (GCA) for 6 h after an initial 3 h attachment period. LDH was measured as an indicator of cell death (n = 3; *p < 0.05 vs. control by analysis of variance)