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. 2016 Feb 24;11(2):e0150134. doi: 10.1371/journal.pone.0150134

Fig 4. Vitamin D limits ERK1/2 phosphorylation in the resting chicken CD3+ T cells.

Fig 4

Following incubation of splenocytes with Vitamin D or vehicle only for 4hrs, phosphorylation of ERK1/2 (T202/Y204) was evaluated in CD3+ T cells in the resting cells (unstimulated) or cells stimulated with PMA for 5 minutes using flow cytometry. The data represents flow cytometry analysis of ERK 1/2 phosphorylation in cells treated with vehicle (thick line), Vitamin D (thin line) or Isotype control (grey area) in the resting cells and PMA-stimulated T cells.