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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: ACS Chem Biol. 2015 Nov 4;11(1):31–37. doi: 10.1021/acschembio.5b00779

Figure 2.

Figure 2

A) Schematic showing the functionality of the orthogonal ribosome-controlled fluorescent reporter in the absence (left panel) and presence (right panel) of aminoglycoside. The 0-16S rRNA is shown in black, 0-SD/O-ASD pair is shown in red, tetR mRNA and TetR protein are shown in cyan, PLtet0-1 is shown in blue, the gfp-uv gene and GFP protein are shown in green, and aminoglycoside is shown as a red hexagon. B) Cell pellet fluorescence and fluorescence quantification of E. coli DHSa cells transformed with individual and combined functional elements of the orthogonal ribosome-controlled fluorescent reporter.