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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Gastroenterology. 2015 Nov 12;150(3):707–719. doi: 10.1053/j.gastro.2015.11.002

Figure 5. Induction of NANOG, pSTAT3 and TWIST1 in HCFD and HCFD+LPS NS5A Tg cohorts.

Figure 5

Confocal immunofluorescence (IF) microscopy demonstrated co-localization of TWIST1 with (A) NANOG and (B) pSTAT3 (C) Co-localization of pSTAT3 with NANOG in tumors obtained from HCFD and HCFD+LPS NS5A Tg liver specimens; this immunoreactivity is completely absent in low fat diet liver tissues (magnification 40x oil; n=15 samples/cohort; n=3). Quantifications of the IF data was done using Metamorph software. The scale bar equals 50 μm.