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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Methods. 2015 Aug 24;99:128–134. doi: 10.1016/j.ymeth.2015.08.014

Figure 1. Immunoblot analysis of smooth muscle marker expression in sphincteric and non-sphincteric bioengineered human smooth muscle constructs.

Figure 1

A) Bioengineered human pylorus and SI SMC constructs formed concentric rings by day 4. Immunoblot analyses were performed on bioengineered human SMC constructs after 10 days in culture. B) Positive expression of SMC markers Caldesmon, Smoothelin and SMA was observed in all constructs tested. β-actin served as a loading control. C) Higher expression of the contractile SMC marker smoothelin was observed in bioengineered 3D constructs compared to 2D monolayer cultures.