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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Methods. 2015 Aug 24;99:128–134. doi: 10.1016/j.ymeth.2015.08.014

Figure 4. Physiologic assessment of force generation by bioengineered smooth muscle constructs in response to potassium chloride and acetylcholine.

Figure 4

Force of contraction measurements were acquired by real time force generation measurements taken after stimulation of constructs with 60 mM KCl or 1 µM ACh. A) Representative tracings of human pylorus SMC constructs are presented. Bioengineered human pylorus constructs produced a robust contraction in response to KCl averaging 519.8 µN (SEM±19.97). B) Human SI SMC constructs also contracted in response to KCl averaging 411.8 µN (SEM±32.48). C) Contraction in response to KCl was significantly higher in bioengineered human pylorus constructs compared to SI constructs (*p<0.05, n=3). D–F) Both bioengineered human pylorus and SI SMC constructs contracted in response to ACh with maximum contractions averaging 184.9 µN (SEM±5.66) and 172.1 µN (SEM±2.37), respectively (n=3). Contraction in response to ACh was abrogated with the addition of a muscarinic receptor inhibitor (atropine, red traces).