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. Author manuscript; available in PMC: 2016 Feb 24.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Jan 6;470(2):453–459. doi: 10.1016/j.bbrc.2016.01.013

Fig. 3.

Fig. 3

Alterations of SIRT1, HIF-1α, ac-H3K14 and the cell cycle after si-SIRT1 treatment. Western blot and quantification of SIRT1 (A) and HIF-1α (B) protein after MPP+ treatment, siRNA transfection or both. (C) qRT-PCR assay used to test HIF-1α changes at the mRNA level. (D) A specific H3K14-ac antibody was applied to detect the H3K14-ac level after different treatments (E) *P < 0.05, **P < 0.01, ***P < 0.001, compared to controls (a), MPP+ treatment only (b) or si-SIRT1 only (c). Flow cytometry (F) and quantification (G) of the stage of cell cycle after different treatment was analyzed. Data are expressed as mean ± standard error.