Fig. 5. The measurement of phosphorylation of p53 level in N2A cells under high glucose condition and agmatine treatment. (A, B) p53 phosphorylation levels were measured by using western blotting. In agmatine treatment group, the protein level of p-p53 was increased compared to the normal control group. The high glucose injured group showed lesser protein levels of p-p53 than normal control group. In high glucose condition, agmatine treatment increased the phosphorylation of p53 in the cells. Data were expressed as mean±S.E.M, and each experiment included 3 repeats per condition. beta-actin was used as an internal control. Differences were considered significant at ^*p<0.05, ^**p<0.001.