Table 1. Bacterial strains and plasmids used in this study.
| Designation | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| Xanthomonas vesicatoria | ||
| 17 | Wild-type, Rif R | This study |
| fur-m | fur mutant strain, containing truncated fur gene and Gm cassette, Rif R, GmR | This study |
| fur-c | fur complementation strain, fur-m containing pMLfur, RifR, KmR, GmR | This study |
| Escherichia coli | ||
| DH5α | Competent cells, Φ80 lacZ | TaKaRa (China) |
| pRK600 | Helper strain in triparental matings, CmR | [18] |
| Agrobacterium tumefaciens | ||
| NTL4 | Quorum-sensing reporter strain, containing traG::lacZ, tra reporter, GmR | [55] |
| Erwinia carotovora subsp. carotovora | ||
| 3 | Indicator strain for quorum sensing | This study |
| Plasmids | ||
| PMD18-T | T-vector, ApR | TaKaRa (China) |
| pK18mob | Cloning and suicide vector with a sacB gene, KmR | [17] |
| pKfur | pK18mob containing a 1572-bp fragment containing fur and its 3’ and 5’ sequences, KmR | This study |
| pKfurGm | pK18 containing wild-type fur with its 209-bp replaced by Gm cassette, KmR, GmR | This study |
| pML123 | Complementation plasmid, Km R, GmR | [16] |
| pMLfur | pML123 containing a 618-bp fragment of fur and its promoter region, KmR, GmR | This study |
aRifR, ApR, KmR, GmR and CmR indicate resistance to rifampicin, ampicillin, kanamycin, gentamicin and chloramphenicol, respectively.