Fig. 2.

Inactivation of GC-A in lung endothelial cells of EC GC-A KO mice. a Effects of ANP on intracellular cGMP content of MLEC prepared from EC GC-A KO and control littermates (10 min incubation; n = 6 per genotype). Inset Representative western blot of GC-A expression in MLEC. b Immunoblot analyses of GC-A expression levels in whole lung protein extracts prepared from EC GC-A KO and control mice (n = 5). c Guanylyl cyclase activity assays: ANP-dependent cGMP synthesis by lung cell membranes prepared from EC GC-A KO and control mice (n = 6). *P < 0.05 vs. controls