Figure 2.
The attachment of platelet lysate-expanded bone marrow mesenchymal stem cells on orthoss scaffold. BM MSCs at passage 2 were incubated with the scaffold by continuous mixing at 37 °C and 5% CO2 for 3 h with DMEM + 50 mL/L PL. The scaffold was then washed and incubated for 10 d with DMEM + 50 mL/L PL. SEM image illustrate MSCs on the scaffold (A). Higher magnification demonstrates MSC morphology on the scaffold surface (B). Black arrow: MSC cells; Red arrow: Orthoss scaffold. BM: Bone marrow; MSCs: Mesenchymal stem cells; PL: Platelet lysate.