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. 2016 Feb 25;3:15. doi: 10.3389/fvets.2016.00015

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Copyright © 2016 Coufal-Majewski, Stanford, McAllister, Blakley, McKinnon, Chaves and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Retention times of ergometrine (8.05 min), ergosine (13.26 min), ergotamine (13.79 min), ergocornine (14.68 min), ergocryptine (15.21 min), ergocristine (15.45 min), and ergosinine (15.86 min) from LC–MS consisting a Agilent 1100 HPLC system with Agilent Zorbax Eclipse XDB-C18 narrow bore 2.1 mm × 150 mm, 5 μm HPLC column and a Quattro Ultima Pt mass spectrometer. The analysis uses mixture of acetonitrile (85%) and 10 mM ammonium acetate (15%) as sample extraction solvent and 10 mM ammonium acetate as mobile phase A and acetonitrile as mobile phase B with the same analytic conditions as described by Krska et al. (42).