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. 2016 Feb 24;11(2):e0150132. doi: 10.1371/journal.pone.0150132

Fig 4. Integrin αvβ3 activity is required for Sdc1-mediated ECM fiber alignment.

Fig 4

A-C) Silencing the expression of the integrin β3 subunit by siRNA diminishes ECM fiber alignment induced by Sdc1. A) Western blot analysis of integrin β3 subunit in HMF mock and HMF Sdc1 cells after treatment with control or β3 siRNA (125nM) for 3 days. B) Representative confocal microscopy images of ECMs labeled with an antibody to fibronectin (FN). The ECMs were derived from HMF-mock or HMF-Sdc1 cells treated with control or β3 siRNA. Original magnification: 200x. C) Mean fiber-to-fiber angles of ECMs produced by HMFs as indicated. Columns labeled with different letters are significantly different (at least p<0.01). D-E) Inhibition of integrin αvβ3 activity in HMF Sdc1 cells by function-blocking antibody LM609 significantly reduces FN fiber alignment. D) Representative confocal images of ECMs labeled with an antibody to FN. The ECMs were produced by HMF-mock, HMF-Sdc1 and HMF-Sdc1 treated with LM609 (30μg/ml). E) Mean fiber-to-fiber angles of HMF-derived ECMs as indicated. Columns labeled with different letters are significantly different (at least p<0.01). Ctrl si, control siRNA; β3 si, β3 siRNA.