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. 2016 Feb 25;7:225. doi: 10.3389/fpls.2016.00225

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Copyright © 2016 Kilgore, Augustin, May, Crow and Kutchan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LC-MS/MS Enhanced Product Ion (EPI) scan of sodium borohydride (NaBH₄) treated CYP96T1 assays with 4′-O-methylnorbelladine substrate. (A) Chromatograph with the following sample runs top to bottom: N-demethylgalanthamine standard, CYP96T1 assay, CPR assay, assay with no Sf9 cells and CYP96T1 assay without 4'-O-methylnorbelladine. (B) EPI fragmentation pattern of the N-demethylgalanthamine standard peak eluting at 4 min. (C) EPI fragmentation pattern of the N-demethylgalanthamine product in the CYP96T1 assay. (D) EPI fragmentation pattern of epi-N-demethylgalanthamine from the CYP96T1 assay. (E) EPI fragmentation pattern of (10bS,4aR)- and (10bR,4aS)-noroxomaritidine standard reduced to stereoisomeric 8-O-demethylmaritidine. (F) EPI fragmentation pattern of reduced (10bS,4aR)- and (10bR,4aS)-noroxomaritidine product from CYP96T1 assays.