Table 2.
MS/MS parameters for substrate tests.
| Substrate | Product specific parameters (Collision energy) (Declustering potential) (First quadrupole m/z) | Substrate specific parameters (Collision energy) (Declustering potential) (First quadrupole m/z) |
|---|---|---|
| 4′-O-Methylnorbelladine | (35)(70)(272.30) | (20)(60)(274.30) |
| 4′-O-Methyl-N-methylnorbelladine | (35)(70)(286.20) | (20)(60)(288.30) |
| 3′-O-Methylnorbelladine | (35)(70)(272.30) | (35)(60)(274.30) |
| 3′,4′-O-Dimethylnorbelladine | (35)(70)(286.20) | (20)(60)(288.30) |
| Norbelladine | (35)(60)(258.00) | (15)(50)(260.00) |
| N-Methylnorbelladine | (35)(70)(272.30) | (20)(60)(274.30) |
| Haemanthamine | (35)(70)(300.12)/(35)(70) (318.13)HO | (35)(70)(302.14) |
| (10bS,4aR)- and (10bR,4aS)-Noroxomaritidine | (35)(70)(270.30)/(35)(70) (288.30)HO | (35)(70)(272.30) |
| Isovanillin and tyramine | (20)(40)(290.30)a/(20)(60)(272.20)b/(35)(70)(270.20)c | (20)(60)(138.20)/(20)(50)(153.20) |
| (S)-Coclaurine | (35)(70)(284.30)/(30)(60) (570.60)dim | (20)(70)(286.30) |
| (R)-Coclaurine | (35)(70)(284.30)/(30)(60) (570.60)dim | (20)(70)(286.30) |
| 4′-O-Methylnorbelladine assays followed by sodium borohydride derivatization | (20)(60)(274.30) | (20)(60)(274.30) |
HO
hydroxylation monitored;
dim
dimer formation monitored;
a
C-C phenol coupling with no amine aldehyde condensation;
b
amine aldehyde condensation/amine aldehyde condensation with C-C phenol coupling and a reduction;
c
amine aldehyde condensation with C-C phenol coupling.