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. 2016 Feb 25;7:171. doi: 10.3389/fpls.2016.00171

FIGURE 1.

FIGURE 1

Schematic representation of Apple latent spherical virus (ALSV) binary vectors and comparison of their capability to induce RNA silencing at their three cloning sites. (A) Genetic map of ALSV binary vector (pBCALR1-SM and pBCALR2-XSB/MN). LB and RB, left and right borders, respectively; P35S, CaMV 35S RNA promoter; NOS, nopaline synthase terminator. The open reading frames of ALSV represent the protease co-factor (Pro-co), NTP-binding helicase (HEL), cysteine protease (C-Pro), RNA polymerase (POL), movement protein (MP), and three capsid proteins (Vp25, Vp20, and Vp24). (B) Comparison of RNA silencing efficiency for the three cloning sites of ALSV vectors with a fragment of the MdrbcS gene inserted. The relative expression of MdrbcS-mRNA was estimated by quantitative RT-PCR.