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RT-PCR detection of ALSV and ACLSV from apple seedlings before and after high-temperature treatment. (A–C) Detection of ALSV and ACLSV from apple seedlings grown at 25°C (A), then grown at 37°C for 4 weeks (wks; B), and transferred to 25°C and grown for 2 months (mo; plant nos. 1–9) in (C). (D) Quantitative RT-PCR of ALSV from apple seedlings (plant nos. 1–9) in (C).
