Table 1.

Infection rate of apple latent spherical virus (ALSV) vectors to apple, pear, and Japanese pear following biolistic inoculation^∗.

Plants	Inoculation sites (plant stages)	Infection rate (%)
		Local (cotyledons)	Systemic (true leaves)
Apple	Cotyledon (seed) ∗∗	100	90–100
Pear	Cotyledon (seed)	100	70
Japanese pear	Cotyledon (seed)	100	0–20
Apple	True leaf (2nd leaf stage)∗∗∗	100	50
	True leaf (3rd leaf stage)	100	20–50
	True leaf (4th leaf stage)	100	20

^∗Experiments were repeated at least twice (at least six plants per experiment). wtALSV and ALSV-AtFT/MdTFL1 were used as inocula. Virus was detected by RT-PCR at 7 days post inoculation (dpi) for inoculated leaves and at 30 dpi for upper true leaves. ^∗∗Cotyledons just after germination were inoculated with ALSV-RNAs. ^∗∗∗First leaf at second leaf stage, first and second leaves at third leaf stage, and first to third leaves at fourth leaf stage were inoculated with ALSV-RNAs.