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LC-MS results of SIRT1 activation. LC-MS chromatograms in negative ion mode and structures of (a) substrate TPE-GK(Ac)YDD and (b) deacetylated product TPE-GKYDD. (c) Area of deacetylated product in LC-MS chromatogram. SIRT1 was incubated with or without ginsenoside Rb2 (Rb2), ginsenoside F1 (F1), ginsenoside Rc (Rc), and schisandrin A (Sch A). Each bar represented the mean ± SD of triplicate experiments. Compared with control group, ∗∗ p < 0.01.
