Abstract
Three methods for rapidly detecting beta-lactamase activity in Haemophilus influenzae are compared. The chromogenic cephalosporin method was found to be the most easily performed and the reagents could be stored for up to three weeks. The phenol red method was simple to perform but the iodometric method was more time consuming. All three tests gave identical results.
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Selected References
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- Catlin B. W. Iodometric detection of Haemophilus influenzae beta-lactamase: rapid presumptive test for ampicillin resistance. Antimicrob Agents Chemother. 1975 Mar;7(3):265–270. doi: 10.1128/aac.7.3.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Escamilla J. Susceptibility of Haemophilus influenza to ampicillin as determined by use of a modified, one-minute beta-lactamase test. Antimicrob Agents Chemother. 1976 Jan;9(1):196–198. doi: 10.1128/aac.9.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kammer R. B., Preston D. A., Turner J. R., Hawley L. C. Rapid detection of ampicillin-resistant Haemophilus influenzae and their susceptibility to sixteen antibiotics. Antimicrob Agents Chemother. 1975 Jul;8(1):91–94. doi: 10.1128/aac.8.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Medeiros A. A., O'Brien T. F. Ampicillin-resistant Haemophilus influenzae type B possessing a TEM-type beta-lactamase but little permeability barrier to ampicillin. Lancet. 1975 Mar 29;1(7909):716–719. doi: 10.1016/s0140-6736(75)91630-x. [DOI] [PubMed] [Google Scholar]
- O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PERRET C. J. Iodometric assay of penicillinase. Nature. 1954 Nov 27;174(4439):1012–1013. doi: 10.1038/1741012a0. [DOI] [PubMed] [Google Scholar]
- Thomas W. J., McReynolds J. W., Mock C. R., Bailey D. W. Letter: Ampicillin-resistant Haemophilus influenzae meningitis. Lancet. 1974 Feb 23;1(7852):313–313. doi: 10.1016/s0140-6736(74)92617-8. [DOI] [PubMed] [Google Scholar]
- Williams J. D., Cavanagh P. Letter: Ampicillin-resistant Haemophilus influenzae meningitis. Lancet. 1974 May 4;1(7862):864–864. doi: 10.1016/s0140-6736(74)90508-x. [DOI] [PubMed] [Google Scholar]
