Fig 1. Flow chart documenting the series of BLAST searches and filtering leading from the initial 5,970 ORFs detected from the de novo assembly of the STIB900 T. b. rhodesiense genome to the final two genes specific to this Tbr strain.

Of note is the high number of variant surface glycoprotein (VSG) genes, which show substantial specificity to the T. b. rhodesiense strain and were detected at each level of filtering.