Brown preadipocytes were transfected with scramble or LNA‐antimiR‐455 at 85% confluency. Two days after transfection, the cells were induced to differentiate by standard differentiation protocol (see
Materials and Methods). Cells were harvested at the indicated time points. Oil Red O staining of the cells on day 8 is shown in (A). The expression levels of miR‐455 (B), its target genes (C–E) and adipogenic marker genes (F–L) were analyzed by qRT–PCR. Data were analyzed with Student's
t‐test and are presented as mean ± SEM of a representative of three independent experiments each performed in quadruplicates (*
P < 0.05 and **
P < 0.01).
