Brown preadipocytes established from PGC1α WT and KO mice were transduced with control lenti‐vector or lentimiR‐455 viruses, selected, pooled, and induced to differentiate by standard differentiation protocol (see
Materials and Methods). On day 8, cells were harvested and analyzed. Shown are qRT–PCR analysis of miR‐455 expression (A), Oil Red O staining (B), and qRT–PCR analysis of adipogenic gene expression (C). Data were analyzed with Student's
t‐test and are presented as mean ± SEM of a representative from three independent experiments each performed in triplicates (*
P < 0.05, **
P < 0.01, and ***
P < 0.001; n.s., non‐significant).
