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. 2016 Feb 25;6:21972. doi: 10.1038/srep21972

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(A) cleaved caspase-3, mitochondrial (Mito) Bax, and cytosolic (Cyto) cytochrome C were analyzed by immunoblotting. CoxIV and β-actin served as loading controls. (B–D). Semi-quantitative analysis of cleaved caspase-3 (B) mitochondrial Bax (C) and cytosolic cytochrome C (D). ^*P < 0.05 vs. the corresponding sham group; ^#P < 0.05 vs. the WT-MI group, n = 5 in each group. (E) Immunohistochemistry of cleaved caspase-3 in the remote non-infarct area in each group. Scale bar, 50 μm.