Figure 2. Performance benchmark.

(a) Figures of merit for CSSSTORM (red), FALCON (blue) and SPIDER (black). The simulation images correspond to an average photon number of 5,000 per molecule (standard deviation of 2,000) and a background of 100 photons with a Poisson distribution (see Methods section); the width of the PSF of the emitters is 390 nm and pixels have a size of 166 nm. Calculations were repeated in 50-fold; (b) Comparison of the reliability of CSSTORM, FALCON and SPIDER for a simulation map of randomly placed emitters with a density 15 μm⁻² (benchmark results shown in Fig. 2a), corresponding to 240 molecules on a 24 by 24 pixel map (red crosses and black dots mark true and estimated positions, respectively).