Figure 3. Results obtained for a series of three juxtaposed circles with a diameter of 400, 600 and 800 nm, respectively.

The emitters are positioned every 20 nm on the circles with a τ_off/τ_on ratio of 31, leading to an average of 18 active fluorophores per frame (fluorophore density of 15 μm⁻²). We report in (a) the average image over 300 frames of the simulated data and the results of (b) CSSTORM; (c) FALCON and (d) SPIDER. The results show that CSSTORM show some spatial distortion in the position of the emitters, while FALCON and SPIDER allow the structure to be recovered reliably (for more details, see Supplementary Fig. S3).