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. 2016 Feb 25;6:21413. doi: 10.1038/srep21413

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The different images are reconstructed at different times and for a time sampling ranging from 3 s (averaging of SPIDER density estimations over 100 frames) down to 0.5 s (averaging of SPIDER density estimations over 15 frames). It shows that increasing time sampling for live-cell imaging super-resolution can provide enhancement of the spatial signal and allows following structural and morphological changes.